A variety of rabies vaccines

The advent of rabies vaccines has saved many people from getting rabies vaccines to protect them from the virus.What are the different kinds of rabies vaccine？Rabies Vaccine After Animal Bite What's the reaction？

Purified Vero cell vaccine

purified Vero cell Rabies vaccine (PVRV) plays an important role in Rabies Prevention. The following is a detailed analysis of the purified Vero cell vaccine:

1. Vaccine background
History: The purified Vero cell vaccine has been used in rabies prevention for many years, and its production and use are widely recognized.
Status: Vero cells are vaccine production cell lines recognized by the World Health Organization and China's biologics Regulations and are widely used in the production of viral vaccines, including rabies vaccines.
Ii. Vaccine characteristics
High efficiency:
PVRV is highly immunogenic and can stimulate the body to produce enough rabies virus neutralizing antibodies (RVNA) to effectively prevent the occurrence of rabies.
Studies have shown that PVRV inoculation before and after exposure can achieve good immune effects.
Security:
PVRV has undergone strict production process and quality control to ensure the safety and stability of the vaccine.
Clinical trials and practical applications have proved that PVRV causes few adverse events during vaccination, and most of them are mild and short-lived.
Easy to produce:
Vero cells have the characteristics of strong stability, strong proliferation and easy cultivation, which makes the production of PVRV more efficient and convenient.
The production process of PVRV is relatively simple, the cost is low, and it is suitable for large-scale production and use.

Vaccine application
Vaccination schedule:
PVRV vaccination protocols can be adjusted according to different situations, including Zagreb protocols and Essen protocols. These regimens performed well in terms of immunogenicity and safety.
In some special populations, such as disease patients or immunocompromised people, PVRV also has a good immune effect and safety.
Combined vaccination:
The combination of PVRV and other vaccines (such as adsorbed acellular DPT inactivated polio combined vaccine, etc.) did not detect immune interference, which provided the feasibility of rabies vaccination in children when receiving immunization program vaccines.
Global Application:
PVRV has been widely used in the world and has made important contributions to the prevention and control of rabies. Especially in areas with severe rabies prevalence, PVRV vaccination is of great significance in reducing the incidence and mortality of rabies.

Human diploid cell vaccine

Human Diploid Cell Vaccine (HDCV) is a kind of vaccine cultured with human diploid cells as a matrix, which has many advantages, especially in the field of rabies vaccine. The following is a detailed analysis of the human diploid cell vaccine:
1. Vaccine background
Definition: Human diploid cell vaccine refers to the use of human diploid cells (such as WI-38, MRC-5 and other cell lines) as the matrix, through culture, harvest, inactivated or attenuated vaccines and other processes.
Characteristics: Human diploid cell vaccine is considered to be a safe and effective vaccine because its stromal cells are derived from human body and have better biocompatibility and lower sensitization.
2. Advantages of vaccines
High safety: human diploid cell vaccine has no carcinogenicity, no contamination by foreign factors, and stable cell properties, reducing the occurrence of adverse reactions after inoculation.
Good immunogenicity: it can stimulate the body to produce a strong immune response, produce enough neutralizing antibodies, and effectively prevent disease.
Fast rate of effect: After inoculation, antibodies can be quickly produced in the body to provide timely protection.
Long duration of immunity: The immune memory generated after vaccination can last for a long time, providing long-term protection for the vaccinated person.

Vaccine application
Rabies vaccine: Human diploid cell rabies vaccine is recommended by the World Health Organization (WHO) as the standard vaccine for evaluating other types of human rabies vaccines, and is the internationally recognized gold standard vaccine for rabies prevention. It has the advantages of high safety, good immunogenicity, fast rate of onset, long duration of immunity and low incidence of adverse reactions.
Other vaccines: In addition to rabies vaccines, human diploid cells are also used in the development and production of other vaccines, such as chickenpox vaccine, rubella vaccine and other inactivated vaccines.
Iv. Vaccine preparation
Preparation process: The preparation process of human diploid cell vaccine includes cell culture, virus inoculation, virus harvest, inactivation or attenuated, purification and other steps. Cell culture is one of the key processes to ensure that cells are grown in a sterile, non-toxic environment.
Quality control: In the preparation process, the quality of each link needs to be strictly controlled to ensure the safety and effectiveness of the vaccine. At the same time, it is also necessary to carry out strict testing and evaluation, including the detection of virus titer, purity, safety and so on.

Primary cell culture vaccine

Primary cell culture vaccine is a method in which primary cells isolated from living tissues or organs are cultured and used to prepare vaccines. This vaccine has certain application in the prevention of rabies and other diseases. The following is a detailed analysis of the primary cell culture vaccine:

I. Definition and characteristics
Definition: Primary cell culture vaccine refers to the fresh tissues or organs of animals after cutting, digestion, dispersion, culture under appropriate conditions, the first generation of cells obtained (that is, primary cells) for the preparation of vaccines.
Features:
Direct source: Primary cells are directly derived from living tissues or organs, maintaining high biological activity and similarity.
High safety: Since the cells are derived from the organism itself, the risk of contamination by external factors is reduced.
Strong immunogenicity: primary cells can express antigens in a variety of organisms, thus stimulating the body to produce a strong immune response.

Preparation process
Tissue harvesting: The desired tissue or organ is removed from a live animal and initial treatment is performed to remove impurities and blood clots.
Cell separation: The tissue is dispersed into individual cells by mechanical method or enzymatic hydrolysis, commonly used enzymes including trypsin.
Cell culture: The isolated primary cells were inoculated into an appropriate culture container, adding an appropriate amount of media, and cultured at a sterile, appropriate temperature and under certain conditions.
Vaccine preparation: When the cells grow to a certain number, vaccines can be prepared by inactivation or attenuated methods.
3. Application and advantages
Application: Primary cell culture vaccine has certain application in the prevention of rabies and other diseases. For example, gopher kidney cells can be used in the production of rabies vaccines.
Advantages:
High efficiency: Primary cells can efficiently express antigens in organisms, thereby improving the immune effect of vaccines.
Safety: Because the cells are derived from the organism itself, and after strict screening and treatment, the safety of the vaccine is high.
Wide adaptability: Primary cell culture vaccines can be prepared against different kinds of viruses and have a wide range of adaptability.